A process by which vegetative organisms, excluding spores, are killed in order to prevent the items disinfected from passing on infection. Equipment, bedlinen and hard surfaces may all be disinfected – the method chosen will depend on the material and size of the object. One of the most important procedures in preventing the spread of infection is the careful washing of hands before handling equipment and between treating different patients. STERILISATION is different from disinfection in that the methods used kill all living organisms and spores.
(1) Skin, wounds, etc. – chlorhexidine (with detergent or spirit); iodine (with detergent or spirit); cetrimide; ethyl alcohol; all must stay in contact with the skin for long enough for bacteria to be killed. (2) Hard surfaces (floors, walls, etc.) – hypochlorites (i.e. bleaches) with or without detergent; cetrimide; iodine-containing solutions; ethyl alcohol. (3) Equipment – wet or dry heat (e.g. boiling for more than 5 minutes); submersion in liquid disinfectants for the appropriate time (e.g. glutaraldehyde 2.5 per cent), chlorhexidine in spirit 70 per cent, formaldehyde (irritant), chlorhexidine (0·1 per cent aqueous), hypochlorites.